Thanks for the fascination In this particular product. Remember to provide us using your Get in touch with facts and your local representative will Call you using a custom made quote. Wherever suitable, they could also guide you having a(n): Believed shipping time for the spot Products sample or unique present In-lab demonstration By submitting this type, you're delivering your consent to STEMCELL Technologies copyright Inc.
RNPs are sent to CD34+ cells via the CellPore™ Transfection System. Put up-transfection, cells are returned to culture and subsequently analyzed for editing effectiveness by using founded strategies as permitted via the experimental design and style, which include movement cytometry or ArciTect™ T7 Endonuclease I Kit (Catalog #76022). Edited progenitor cell functionality could be calculated by in vitro colony-forming device (CFU) assays or cells could be even more cultured for lineage-specific differentiation utilizing tailor-made StemSpan™ media and health supplements.
Measure the prospective toxic consequences of applicant therapeutics, including small molecule compounds and biologics.
Operate immediately at this step to pool wells right into a 15 mL tube. Note: For a significant-scale dissociation, use a multichannel pipette to pool cells right into a sterile reagent reservoir. Increase the pooled wells into a fifteen mL tube.
This product or service is designed for use in the next research location(s) as A part of the highlighted workflow stage(s). Take a look at these workflows To find out more about the other solutions we provide to assistance Each and every investigate space. Study Spot Workflow Levels
Make a lot more informed purchasing conclusions with our new merchandise availability and shipping and delivery estimate element, now offered on all solution pages, as part of your cart, and during checkout.
There are many commercially out there sources for Principal airway epithelial cells. We have 가슴수술 now tested HAECs from two sellers, Lonza and Epithelix — the two have resulted in effective ALI cultures.
Note: Move forward to antibody staining if undertaking live cell Evaluation soon after this action. Alternatively, proceed to fixable dye staining and 줄기세포 지방이식 PFA fixation.
With this review, We'll give attention to the complexity of memory B cells as well as their role in infectious ailments, and may emphasize new developments in the sector. Examine More
New to STEMCELL? Sign up for an Stem cell account to rapidly and simply buy products and solutions 가슴수술 on the internet and for one particular-click access to all instructional stem cell clinic information. Generate an Account
When coming up with information RNAs, it is suggested to select sequences that align Along with the transcription start out internet site in the gene of interest where by doable.
A minimal reaction volume of 50 µL is needed for regular performance While using the CellPore™ Transfection System.
Thank you for the interest in this product. Please deliver us with your Call information and facts and your neighborhood consultant will Make contact with you having a customized quotation. Wherever acceptable, they also can help you with a(n): Believed shipping time for the area Products sample or distinctive supply In-lab demonstration By publishing this way, you might be supplying your consent to STEMCELL Systems copyright Inc.
The following fluorochrome-conjugated antibodies and dyes are recommended to aid Investigation of gene-edited CD34+ HSPCs: